BBa_K2110827

BBa_K2110827 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_K2110827

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Haipeng Yu

Date created: 2016-10-04 11:00:00

Date modified: 2016-10-11 07:42:01

PETase site mutation S207T - F

Details

• Types: DnaRegion
• Roles: primer; Primer
• Sequences: BBa_K2110827_sequence (Version 1)

Description

This is the forward for mutant PETase. Site S207 was designed to be replaced by T207. The original codon optimized sequence codes the wild type protein called PETase. PETase is a Poly(ethylene terephthalate) hydrolase, which was found in Ideonella sakaiensis (strain 201-F6) by Japanese scientists. It allows Ideonella sakaiensis to degrade PET. If properly transferred into engineered organisms as E.Coli or yeast, secreted PETase can catalyze the hydrolysis of PET to produce mono(2-hydroxyethyl) terephthalate (MHET) as the major product. Optimum temperature is 40 degrees Celsius for PET film hydrolysis and optimum pH is 9.

Notes

First apply PCR for mutant section. Then PCR again with original primers of PETase to overlap for the entire mutant PETase.

Source

Artificially Designed

Other Properties

• igem#experience: None
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2110827/1

Member of these Collections

• iGEM Parts Registry