Types | DnaRegion
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Roles | CDS
Coding
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Description
C-terminal fusion of anti-GFP nanobody to spore crust gene cotZ by a flexible GGGGS linker.
Notes
Introduction of HA epitope tag and GGGGS linker by primer extensions and PCR.
Source
The cotZ gene comes from the genome of B. subtilis. The anti-GFP nanobody is derived from camelid heavy-chain antibodies.