Types | DnaRegion
|
Roles | Coding
CDS
|
Description
C-terminal fusion of anti-GFP nanobody to spore crust gene cotZ by an alpha helical linker.
Notes
Introduction of HA epitope tag and alpha helical linker by primer extensions and PCR.
Source
The cotZ gene comes from the genome of B. subtilis. The anti-GFP nanobody is derived from camelid heavy-chain antibodies.