BBa_K2114005

BBa_K2114005 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2114005
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wladislaw Stroukov
Date created: 2016-10-07 11:00:00
Date modified: 2016-10-10 08:19:56

GST_HA_G4S_CotZ



    • Details

    Types
    DnaRegion

    Roles
    CDS

    Coding

    Sequences BBa_K2114005_sequence (Version 1)

    Description

    N-terminal fusion of glutathione S-transferase to spore crust gene cotZ by a flexible GGGGS linker.

    Notes

    Introduction of HA epitope tag and flexible GGGGS linker by primer extensions and PCR.

    Source

    The cotZ gene comes from the genome of B. subtilis. The glutathione S-transferase was amplified from the pGEX-6P-1 vector (GE healthcare).

    Sequence Annotation Location Component / Role(s)
    Glutathione S-transferase
    HA
    GGGGS linker
    cotZ
    		1,699
    700,726
    727,741
    742,1188
    		CDS feature/cds
    tag feature/tag
    feature/misc sequence_feature
    feature/cds CDS

    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K2114005/1
     

    Attachments

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