BBa_K2114005

BBa_K2114005 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2114005
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wladislaw Stroukov
Date created: 2016-10-07 11:00:00
Date modified: 2016-10-10 08:19:56

GST_HA_G4S_CotZ



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K2114005_sequence (Version 1)

Description

N-terminal fusion of glutathione S-transferase to spore crust gene cotZ by a flexible GGGGS linker.

Notes

Introduction of HA epitope tag and flexible GGGGS linker by primer extensions and PCR.

Source

The cotZ gene comes from the genome of B. subtilis. The glutathione S-transferase was amplified from the pGEX-6P-1 vector (GE healthcare).

Sequence Annotation Location Component / Role(s)
Glutathione S-transferase
HA
GGGGS linker
cotZ
1,699
700,726
727,741
742,1188
CDS feature/cds
tag feature/tag
feature/misc sequence_feature
feature/cds CDS
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K2114005/1