BBa_K2114006

BBa_K2114006 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2114006
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wladislaw Stroukov
Date created: 2016-10-07 11:00:00
Date modified: 2016-10-10 08:36:01

CotZ_G4S_HA_GST



    • Details

    Types
    DnaRegion

    Roles
    Coding

    CDS

    Sequences BBa_K2114006_sequence (Version 1)

    Description

    C-terminal fusion of glutathione S-transferase to spore crust gene cotZ by a flexible GGGGS linker.

    Notes

    Introduction of HA epitope tag and flexible GGGGS linker by primer extensions and PCR.

    Source

    The cotZ gene comes from the genome of B. subtilis. The glutathione S-transferase was amplified from the pGEX-6P-1 vector (GE healthcare).

    Sequence Annotation Location Component / Role(s)
    cotZ
    GGGGS linker
    HA
    Glutathione S-transferase
    		1,444
    445,459
    460,486
    487,1188
    		CDS feature/cds
    feature/misc sequence_feature
    tag feature/tag
    CDS feature/cds

    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K2114006/1
     

    Attachments

    © 2018 Newcastle University, University of Utah, and collaborators
    About SynBioHub | View Source on Github | Report an Issue
    | v1.6.1 (86615526)