Types | DnaRegion
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Roles | Coding
CDS
|
Description
N-terminal fusion of anti-GFP nanobody to spore crust gene cotG by a flexible GGGGS linker.
Notes
Introduction of HA epitope tag and flexible GGGGS linker by primer extensions and PCR.
Source
The cotG gene comes from the genome of B. subtilis. The anti-GFP nanobody is derived from camelid heavy-chain antibodies.