BBa_K2114010

BBa_K2114010 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2114010

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Wladislaw Stroukov

Date created: 2016-10-07 11:00:00

Date modified: 2016-10-12 01:26:47

GST_HA_G4S_CotG

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K2114010_sequence (Version 1)

Description

N-terminal fusion of glutathione S-transferase to spore crust gene cotG by a flexible GGGGS linker.

Notes

Introduction of HA epitope tag and flexible GGGGS linker by primer extensions and PCR.

Source

The cotG gene comes from the genome of B. subtilis. The glutathione S-transferase gene was amplified from the pGEX-6P-1 vector (GE healthcare).

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
glutathione S-transferase	1,699	CDS, feature/cds
HA	700,726	tag, feature/tag
GGGGS linker	727,741	feature/misc, sequence_feature
cotG	742,1329	feature/cds, CDS

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2114010/1

Member of these Collections

• iGEM Parts Registry