Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wladislaw Stroukov
Date created: 2016-10-07 11:00:00
Date modified: 2016-10-19 03:34:59
CotG_G4S_HA_GST
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K2114011_sequence (Version 1) |
Description
C-terminal fusion of glutathione S-transferase to spore crust gene cotG by a flexible GGGGS linker.Notes
Introduction of HA epitope tag and alpha helical linker by primer extensions and PCR.Source
The cotG gene comes from the genome of B. subtilis. The glutathione S-transferase gene was amplified from the pGEX-6P-1 vector (GE healthcare).| Sequence Annotation | Location | Component / Role(s) |
| cotG GGGGS linker HA glutathione S-transferase | 1,585 586,600 601,627 628,1329 | feature/cds CDS feature/misc sequence_feature feature/tag tag feature/cds CDS |