Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wladislaw Stroukov
Date created: 2016-10-08 11:00:00
Date modified: 2016-10-12 02:23:01
GST_HA_aHelix_CotG
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K2114012_sequence (Version 1) |
Description
N-terminal fusion of glutathione S-transferase to spore crust gene cotG by an alpha helical linker.Notes
Introduction of HA epitope tag and alpha helical linker by primer extensions and PCR.Source
The cotG gene comes from the genome of B. subtilis. The glutathione S-transferase gene was amplified from the pGEX-6P-1 vector (GE healthcare).| Sequence Annotation | Location | Component / Role(s) |
| glutathione S-transferase HA alpha helical linker cotG | 1,699 700,726 727,759 760,1347 | feature/cds CDS feature/tag tag sequence_feature feature/misc feature/cds CDS |