BBa_K2114012

BBa_K2114012 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2114012
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wladislaw Stroukov
Date created: 2016-10-08 11:00:00
Date modified: 2016-10-12 02:23:01

GST_HA_aHelix_CotG



    • Details

    Types
    DnaRegion

    Roles
    CDS

    Coding

    Sequences BBa_K2114012_sequence (Version 1)

    Description

    N-terminal fusion of glutathione S-transferase to spore crust gene cotG by an alpha helical linker.

    Notes

    Introduction of HA epitope tag and alpha helical linker by primer extensions and PCR.

    Source

    The cotG gene comes from the genome of B. subtilis. The glutathione S-transferase gene was amplified from the pGEX-6P-1 vector (GE healthcare).

    Sequence Annotation Location Component / Role(s)
    glutathione S-transferase
    HA
    alpha helical linker
    cotG
    		1,699
    700,726
    727,759
    760,1347
    		feature/cds CDS
    feature/tag tag
    sequence_feature feature/misc
    feature/cds CDS

    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K2114012/1
     

    Attachments

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