BBa_K2114012

BBa_K2114012 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2114012

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Wladislaw Stroukov

Date created: 2016-10-08 11:00:00

Date modified: 2016-10-12 02:23:01

GST_HA_aHelix_CotG

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K2114012_sequence (Version 1)

Description

N-terminal fusion of glutathione S-transferase to spore crust gene cotG by an alpha helical linker.

Notes

Introduction of HA epitope tag and alpha helical linker by primer extensions and PCR.

Source

The cotG gene comes from the genome of B. subtilis. The glutathione S-transferase gene was amplified from the pGEX-6P-1 vector (GE healthcare).

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
glutathione S-transferase	1,699	feature/cds, CDS
HA	700,726	feature/tag, tag
alpha helical linker	727,759	sequence_feature, feature/misc
cotG	760,1347	feature/cds, CDS

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2114012/1

Member of these Collections

• iGEM Parts Registry