BBa_K2114015

BBa_K2114015 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2114015

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Wladislaw Stroukov

Date created: 2016-10-08 11:00:00

Date modified: 2016-10-09 07:30:50

aGFPnano_HA_G4S_CgeA

Details

• Types: DnaRegion
• Roles: CDS; Coding

Description

N-terminal fusion of anti-GFP nanobody to spore crust gene cgeA by a flexible GGGGS linker.

Notes

Introduction of HA epitope tag and flexible GGGGS linker by primer extensions and PCR.

Source

The cgeA gene comes from the genome of B. subtilis. The anti-GFP nanobody is derived from camelid heavy-chain antibodies described by Kubala, M. H., Kovtun, O., Alexandrov, K. & Collins, B. M. Structural and thermodynamic analysis of the GFP:GFP-nanobody complex. Protein Sci. 19, 2389???2401 (2010).

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2114015/1

Member of these Collections

• iGEM Parts Registry