BBa_K2114018

BBa_K2114018 Version 1

Component

This part has been discontinued.

Source:
http://parts.igem.org/Part:BBa_K2114018
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wladislaw Stroukov
Date created: 2016-10-09 11:00:00
Date modified: 2016-10-11 05:11:02

cgeA_aHelix_HA_aGFPnano



    • Details

    Types
    DnaRegion

    Roles
    CDS

    Coding

    Description

    C-terminal fusion of anti-GFP nanobody to spore crust gene cgeA by an alpha helical linker.

    Notes

    Introduction of HA epitope tag and alpha helical linker by primer extensions and PCR.

    Source

    The cgeA gene comes from the genome of B. subtilis. The anti-GFP nanobody is derived from camelid heavy-chain antibodies described by Kubala et al. in 2010. [Ref]

    igem#experience
    None
     
    igem#status
    Deleted
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K2114018/1
     

    Attachments

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