BBa_K2116008

BBa_K2116008 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2116008
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Asli Azizoglu
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-25 12:23:56

AND gate regulated by norR and esaR (three esaboxes,15bp)



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_K2116008_sequence (Version 1)

Description

AND gate constructed by placing three esaboxes after the transcription start site of PnorV. The esaboxes have 15bp of spacing in between.

Notes

...

Source

PnorV comes from E.coli. esabox []

Sequence Annotation Location Component / Role(s)
NorV promoter
NorR binding 3 low affinity
NorR binding 2 low affinity
NorR binding 1 high affinity
sigma54
esabox
Spacer
esabox
Spacer
esabox
1,285
149,158
162,178
189,209
250,282
302,319
320,334
335,352
353,367
368,385
promoter feature/promoter
non_covalent_binding_site feature/binding
feature/binding non_covalent_binding_site
feature/binding non_covalent_binding_site
non_covalent_binding_site feature/binding
non_covalent_binding_site feature/binding
sequence_feature feature/misc
feature/binding non_covalent_binding_site
feature/misc sequence_feature
feature/binding non_covalent_binding_site
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K2116008/1