Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Asli Azizoglu
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-24 07:38:58
AND gate regulated by Nitric Oxide and 3OC6HSL
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K2116011_sequence (Version 1) |
Description
rAND gate created by placing one esabox as roadblock, and one esabox right before the first norR binding site of the PnorV promoter. This second esabox is added to create competitive binding between norR and esaR, and improve AND gate behaviour.Notes
...Source
PnorV from E.coli, esaboxes from [].| Sequence Annotation | Location | Component / Role(s) |
| esabox NorR binding site 3 (low affinity) NorR binding site 2 (low affinity) NorR binding site 1 (high affinity) sigma 54 binding site esabox AND gate | 130,147 148,157 161,177 188,208 249,281 301,318 1,318 | feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site non_covalent_binding_site feature/binding non_covalent_binding_site feature/binding feature/binding non_covalent_binding_site non_covalent_binding_site feature/binding promoter feature/promoter |