BBa_K2116013

BBa_K2116013 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2116013
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Asli Azizoglu
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-24 10:31:05

AND gate p25



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_K2116013_sequence (Version 1)

Description

AND gate constructed by adding an esabox that starts after the -12 binding site for sigma 54, and replaces some of the sigma 54 binding site.

Notes

...

Source

PnorV from E.coli, esabox from [].

Sequence Annotation Location Component / Role(s)
NorV promoter
NorR binding 3 (low affinity)
NorR binding 2 (low affinity)
NorR binding 1 (high affinity)
sigma54 binding site
esabox
spacer
esabox
1,285
149,158
162,178
189,209
250,282
292,309
310,317
318,335
feature/promoter promoter
non_covalent_binding_site feature/binding
non_covalent_binding_site feature/binding
feature/binding non_covalent_binding_site
non_covalent_binding_site feature/binding
feature/binding non_covalent_binding_site
sequence_feature feature/misc
feature/binding non_covalent_binding_site
igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K2116013/1