Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: HongYu Yang
Date created: 2016-10-11 11:00:00
Date modified: 2016-10-20 11:00:01
araC+pBAD+B0032+mazF
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K2120002_sequence (Version 1) |
Description
This killer device is induced by arabinose.Encodes the liable antitoxin to mazF in Bacillus Subtilis. It is used in conjungtion with mazF in Bacillus Subtilis to provide a toxin-antitoxin kill switch in various stressful conditions.This part contains the promoter as well as the coding sequence for the repressor AraC which is transcribed in the opposite direction. (???upstream???) By binding to L(+)-arabinose, AraC changes its conformation. This causes the protein to diffuses from the DNA thereby inducing transcription.We use it to kill the cell which become plasmid-free.Notes
The device is built to test the threshold. our project can not only set a threshold for the concentration of plasmids but also regulate it as we need. And arabinose is regarded as the concentration of plasmid.Source
Bacillus Subtilis| Access | Instance | Definition |
| public public public | BBa_K808000 BBa_B0032 BBa_K2120008 | Ara BBa_B0032 BBa_K2120008 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K808000 BBa_B0032 BBa_K2120008 | 1,1209 1218,1230 1237,1572 | Ara BBa_B0032 BBa_K2120008 |