BBa_K2121001

BBa_K2121001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2121001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jinny van Doorn
Date created: 2016-10-19 11:00:00
Date modified: 2016-10-20 09:13:17

Lambda_Beta'_A6UAS9 (rhizo_red-MAGE)



    • Details

    Types
    DnaRegion

    Roles
    CDS

    Coding

    Sequences BBa_K2121001_sequence (Version 1)

    Description

    Coding sequence for beta recombinase protein homolog.
    For use in red recombineering (eg Multiplex Automated Genome Engineering, MAGE) of Rhizobia species. Identified from homology to the lambda coliphage beta recombinase via BLASTp search. Derived from phage that selectively infects rhizobia spp (S. med).

    Notes

    Elimination of any illegal restriction sites, and codon optimization for expression in S. meliloti.

    Source

    Sinorhizobium medicae (strain WSM419)

    Sequence Annotation Location Component / Role(s)
    cds
    		1,1008
    		CDS feature/cds

    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K2121001/1
     

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