Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Leslie Wang
Date created: 2016-10-20 11:00:00
Date modified: 2016-10-29 09:53:38
AMed_Citrine_T1
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_K2121014_sequence (Version 1) |
Description
Anderson promoter, citrine fluorescence reporter, and T1 terminator cassette for rhizobia promoter characterization with LIC sites flanking the protein CDS.Notes
Strong RBS that elevates citrine expression level in rhizobia.Source
Anderson promoter and T1 terminator from iGEM registry. Citrine sequence from Stephen Dellaporta Lab, Yale University.| Sequence Annotation | Location | Component / Role(s) |
| promoter pJ23111 RBS Upstream LIC cloning site ORF frame LIC downstream cloning site T1_terminator | 1,35 36,39 40,54 55,1476 1477,1491 1528,1596 | feature/promoter promoter ribosome_entry_site feature/rbs sequence_feature feature/misc feature/cds CDS feature/misc sequence_feature stop_codon feature/stop |