BBa_K2121015

BBa_K2121015 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2121015

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Leslie Wang

Date created: 2016-10-20 11:00:00

Date modified: 2016-10-29 09:54:31

AWk_Citrine_T1

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K2121015_sequence (Version 1)

Description

Anderson promoter, citrine fluorescence reporter, and T1 terminator cassette for rhizobia promoter characterization with LIC sites flanking the protein CDS.

Notes

Strong RBS that elevates citrine expression level in rhizobia.

Source

Anderson promoter and T1 terminator from iGEM registry. Citrine sequence from Stephen Dellaporta Lab, Yale University.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
promoter pJ23114	1,35	feature/misc, sequence_feature
RBS	36,39	feature/rbs, ribosome_entry_site
Upstream LIC cloning site	40,54	sequence_feature, feature/misc
citrine	55,1476	feature/cds, CDS
T1 terminator	1513,1581	feature/stop, stop_codon

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2121015/1

Member of these Collections

• iGEM Parts Registry