BBa_K2130003

BBa_K2130003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2130003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Ooi Kean Hean

Date created: 2016-10-18 11:00:00

Date modified: 2016-10-19 01:58:00

ZsGreen(GFP) reporter with 2X dCas9-inducible promoter

Details

• Types: DnaRegion
• Roles: engineered_region; Reporter
• Sequences: BBa_K2130003_sequence (Version 1)

Description

The reporter is regulated by a dCas9-VPR inducible promoter which has 2 Streptococcus pyogenes dCas9 or SAdCas9 binding sites. The reporter is usable in mammalian cells only.

Notes

Two dCas9 binding sites are identicle

Source

Addgene

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
dCas9 binding site	13,38	promoter, feature/promoter
ZsGreen(GFP)	444,1139	feature/cds, CDS
bGH pA	1167,1394	feature/polya, polyA_site
dCas9 binding site	79,104	feature/promoter, promoter
TRE	107,422	promoter, feature/promoter

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2130003/1

Member of these Collections

• iGEM Parts Registry