TetR-pTet

BBa_K2135002 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2135002
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Harley Greene
Date created: 2016-09-26 11:00:00
Date modified: 2016-09-27 11:02:48

TetR-pTet Inducible Promoter



    • Details

    Types
    DnaRegion

    Roles
    Regulatory

    promoter

    Sequences BBa_K2135002_sequence (Version 1)

    Description

    pTet is a constitutive promoter that is repressed by TetR. TetR is removed when aTc is added at various concentrations. This part had TetR included and constitutively produced. Therefore, this part can be used as an inducible promoter, which will vary promoter strength depending on the concentration of aTc added.

    Notes

    No

    Source

    Cut from plasmid found in Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression [Qi et al. , 2013]
    Plasmid #44249 from AddGene

    Sequence Annotation Location Component / Role(s)
    TetR
    pTet
    		1,629
    629,700
    		feature/cds CDS
    feature/promoter promoter

    igem#experience
    Works
     
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K2135002/1
     

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