Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Patrick Diep
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-13 07:09:16
CUP1-GFP Transcriptional Fusion
| Types | DnaRegion |
| Roles | Measurement engineered_region |
| Sequences | BBa_K2137001_sequence (Version 1) |
Description
It was necessary for us to choose promoters that expressed the constructs we were cloning into yeast cells. We wanted to characterize promoter expression with GFP before we decided which promoter to use in our system with the CFP-Hsp104 construct. Furthermore, we needed to induce a [PSI+] state by overexpression of Sup35 in the cell by inserting another copy of Sup35 into the system via a plasmid. Overall, we had two plasmids that we wanted to easily induce at any time.We chose to characterize four main promoters that are commonly found in yeast: Gal1, Adh1, and Cup1.
The CUP1 promoter is inducible by adding copper to the medium (http://labs.biology.ucsd.edu/subramani/documents/121.pdf)
Notes
No known special design considerations.Source
The part was synthesized through IDT and is derived from the Saccharomyces cerevisiae genome.| Sequence Annotation | Location | Component / Role(s) |
| CUP1 Promoter GFP CYC1 Terminator | 1,235 298,1014 1021,1278 | feature/promoter promoter feature/protein CDS stop_codon feature/stop |