Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Patrick Diep
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-13 07:18:52
Gal1-GFP Transcriptional Fusion
| Types | DnaRegion |
| Roles | engineered_region Measurement |
| Sequences | BBa_K2137002_sequence (Version 1) |
Description
It was necessary for us to choose promoters that expressed the constructs we were cloning into yeast cells. We wanted to characterize promoter expression with GFP before we decided which promoter to use in our system with the CFP-Hsp104 construct. Furthermore, we needed to induce a [PSI+] state by overexpression of Sup35 in the cell by inserting another copy of Sup35 into the system via a plasmid. Overall, we had two plasmids that we wanted to easily induce at any time.We chose to characterize four main promoters that are commonly found in yeast: Gal1, Adh1, and Cup1.
Expression of the GAL1 gene in S. cerevisiae is strongly repressed by growth on glucose. It is shown that two sites within the GAL1 promoter mediate glucose repression. (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC361077/pdf/molcellb00045-0329.pdf)
Notes
No special design considerations made.Source
Synthesized from IDT using a Saccharomyces cerevisiae genomic sequence.| Sequence Annotation | Location | Component / Role(s) |
| Gal1 Promoter GFP CYC1 Terminator | 1,442 455,1171 1177,1434 | feature/promoter promoter CDS feature/protein stop_codon feature/stop |