Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Austin Nykiel
Date created: 2016-10-18 11:00:00
Date modified: 2016-10-19 02:13:39
RNA thermometer and RBS (33C)
| Types | DnaRegion |
| Roles | ribosome_entry_site RBS |
| Sequences | BBa_K2138002_sequence (Version 1) |
Description
The RNA thermometer portion of the part is designed to linearize and allow translation of a target protein at thirty-three degrees Celsius. The ribosome binding site was incorporated into the design and selected following the Anderson ribosome binding site format for optimal protein expression and DNA design. The entire part is able to be restricted from the pSB1C3 backbone and ligated with any type of reporter protein to measure temperature regulated expression.Notes
The primary design consideration while designing the DNA sequence was to achieve a melting temperature between thirty degrees Celsius and forty-two degrees Celsius. This was achieved by eliminating as many cytosine and guanine complementary bonds as possible leading to sequences comprised essentially of three different nucleotides.Source
The DNA sequence is comprised of two different segments. The first segment was designed entirely de novo by utilizing Oligoanalyzer3.1 from Integrated DNA Technologies to calculate melting temperature for the complementary RNA strand. The second segment consists of the ribosome binding site which was selected based upon the Anderson ribosome binding site format for strong protein expression.| Sequence Annotation | Location | Component / Role(s) |
| RNA thermometer Promoter Ribosome Binding Site | 60,107 23,59 94,107 | feature/stem_loop stem_loop promoter feature/promoter ribosome_entry_site feature/rbs |