Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: UBC iGEM 2016
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-30 03:57:26
Ptac RBS Endo5a
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K2139005_sequence (Version 1) |
Description
Endo5a Expression CassetteEndo5a catalyzes the conversion of cellulose into smaller fragments by making internal cuts in the cellulose chain. It can be used in conjunction with exoglucanases for the direct conversion of cellulose into monomeric glucose. The polymerization of the protein is monomeric with an optimal temperature of 50 degrees Celsius and an optimal pH ranging from 6 to 8. The genbank ascension number for this protein is HQ657203.1 (DNA), AEB00655.1 (amino acid), and a PDB code of 1VRX. This construct was synthesized for use in Caulobacter Cresentus and is codon optimized to contain a high GC content.
Literature data for this part can be found http://www.sciencedirect.com/science/article/pii/S10465928120031
Notes
Paenibacillus strain ICGEB2008Source
It is codon optimized for Caulobacter cresentus, exhibiting a high GC content.| Access | Instance | Definition |
| public public | BBa_K1813037 BBa_K2139001 | BBa_K1813037 BBa_K2139001 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1813037 BBa_K2139001 | 1,81 88,1281 | BBa_K1813037 BBa_K2139001 |