Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lukas Rieder
Date created: 2016-09-14 11:00:00
Date modified: 2016-10-07 08:33:33
Composite part containing mazE and red chromoprotein
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K2142000_sequence (Version 1) |
Description
This part was used as antitoxin for our mazF toxin. Together mazE and mazF form a functional selectable marker. Further we combined the antitoxin with a red chromoprotein to make visible selection.The part has also a constitutive promoter, A RBS upstream of every coding sequence and an bidirectional double terminated in the end.
Notes
We were working with different antitoxin/toxin systems. To be sure the E.coli will survive its own toxin we had to express the antitoxin constitutive and also the chromoprotein had to be expressed without inducing. The bidirectional double terminator in the end allows us to use only one terminator to safe some space on the plasmid.Source
This part was made with sequences of the registry and from NCBI database.| Access | Instance | Definition |
| public public public public public public | BBa_J23102 BBa_J61100 BBa_K592012 BBa_J61100 BBa_K2142001 BBa_B0024 | BBa_J23102 BBa_J61100 eforRed BBa_J61100 BBa_K2142001 BBa_B0024 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23102 BBa_J61100 BBa_K592012 BBa_J61100 BBa_K2142001 BBa_B0024 | 1,35 44,55 62,742 751,762 769,1017 1026,1120 | BBa_J23102 BBa_J61100 eforRed BBa_J61100 BBa_K2142001 BBa_B0024 |