Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Oskar ??hman
Date created: 2016-09-21 11:00:00
Date modified: 2016-10-19 01:52:24
Coding sequence for Nuclease regulated by T7-promoter
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K2144000_sequence (Version 1) |
Description
Usage:Nuclease is an enzyme with the capability of cleaving phosphodiester bonds between nucleotides in DNA. This enzyme can be used in applications concerning degradation of DNA. In our project the aim was to evaluate and use Nuclease degrading abilities to inhibit the biofilm formation produced by Staphyloccus Auerus.
The encoding part of the BioBrick is derived from BBa_K729004, made by iGEM London 2012. The T7 promoter (BBa_k525998) has been inserted to control protein expession under IPTG induction. To regulate the protein expression under T7 promoter control, E.coli containing a T7 polymerase must be used, for instance BL21(DE3).
Biology:
Nuclease is an extracellular enzyme found in Staphyloccus Aureus. [3] S.Aureus encodes for two Nucleases, Nuc1 and Nuc2, [3] whereof the last-mentioned is a part of this BioBrick. Nuc2 is connected to the membrane with a N-terminal anchor. [3]
There are several bacterial pathogens using Nuclease to combat the immune response in the host by for instance avoiding neutrophil extracellular traps. (NETs). [1] Furthermore, since eDNA is an important component of biofilm [2] Nuc is able to work as a regulator of biofilm formation. [2] Studies have suggested that the expression of Nuc contributes to the inability to produce biofilm [2] and during biofilm formation-conditions the expression of Nuc is repressed. [3]
Notes
The subparts was aligned by 3A Assembly.Source
Nuclease from Staphylococcus Aureus.| Access | Instance | Definition |
| public public | BBa_K525998 BBa_K729004 | pT7 BBa_K729004 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K525998 BBa_K729004 | 1,32 39,599 | pT7 BBa_K729004 |