Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Melody Wu, Laura Xinyu Chen, Katie Maruna
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-30 06:19:00
GFP/RFP reporter (Reverse/Forward) with random 500bp spacer
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_K2145001_sequence (Version 1) |
Description
This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) along with a "spacer" part and pSB1C3 backbone assembled to make the final part. It can be used to express both GFP and RFP proteins.Notes
This part was assembled using Golden Gate Assembly.Source
The part was made from assembling BBa_J04450, BBa__I13522, and pSB1C3.| Sequence Annotation | Location | Component / Role(s) |
| BBa_I13522 500 bp spacer BBa_J04450 | 45,985 986,1485 1490,2558 | feature/BioBrick engineered_region feature/misc sequence_feature feature/BioBrick engineered_region |