BBa_K2145006

BBa_K2145006 Version 1

Component

This part has been discontinued.

Source:
http://parts.igem.org/Part:BBa_K2145006
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Laura Xinyu Chen
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-14 10:10:34

GFP/RFP reporter with spacer part (532bp)



Types
DnaRegion

Roles
Plasmid

plasmid

Description

This plasmid contains a GFP and an RFP reporter separated by a 532-bp spacer containing three gRNA binding sites for dCas. The reporter is designed to demonstrate the effect, if any, of binding a dCas "clamp" (or a random sequence in) between two genes (the idea being that such a clamp can limit propagation of supercoiling between the genes, functionally isolating them).

Notes

Constructed with Golden Gate Assembly.

Source

Constructed from BBa_J04450, BBa_I13522, and a spacer derived from a concatenation of several non-functional plasmid backbone sequences.

igem#experience
None
 
igem#status
Deleted
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K2145006/1