GG97

BBa_K2145104 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2145104
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Laura Xinyu Chen, Melody Wu, Katie Maruna
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-30 06:20:32

GFP/RFP reporter (Forward/Reverse) with random 500bp spacer



Types
DnaRegion

Roles
Reporter

engineered_region

Sequences BBa_K2145104_sequence (Version 1)

Description

This plasmid contains a GFP and an RFP reporter separated by a 532-bp spacer containing three gRNA binding sites for dCas. The reporter is designed to demonstrate the effect, if any, of binding a dCas "clamp" (or a random sequence in) between two genes (the idea being that such a clamp can limit propagation of supercoiling between the genes, functionally isolating them).

Notes

Constructed with Golden Gate Assembly.

Source

Constructed from BBa_J04450, BBa_I13522, and a spacer derived from a concatenation of several non-functional plasmid backbone sequences.

Sequence Annotation Location Component / Role(s)
BBa_I13522
500 bp spacer
BBa_J04450
45,981
986,1485
1490,2558
engineered_region feature/BioBrick
sequence_feature feature/misc
engineered_region feature/BioBrick
igem#experience
Issues
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K2145104/1