Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Laura Xinyu Chen, Melody Wu, Katie Maruna
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-30 06:20:32
GFP/RFP reporter (Forward/Reverse) with random 500bp spacer
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_K2145104_sequence (Version 1) |
Description
This plasmid contains a GFP and an RFP reporter separated by a 532-bp spacer containing three gRNA binding sites for dCas. The reporter is designed to demonstrate the effect, if any, of binding a dCas "clamp" (or a random sequence in) between two genes (the idea being that such a clamp can limit propagation of supercoiling between the genes, functionally isolating them).Notes
Constructed with Golden Gate Assembly.Source
Constructed from BBa_J04450, BBa_I13522, and a spacer derived from a concatenation of several non-functional plasmid backbone sequences.| Sequence Annotation | Location | Component / Role(s) |
| BBa_I13522 500 bp spacer BBa_J04450 | 45,981 986,1485 1490,2558 | engineered_region feature/BioBrick sequence_feature feature/misc engineered_region feature/BioBrick |