GG100

BBa_K2145107 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2145107

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Laura Xinyu Chen, Melody Wu

Date created: 2016-10-13 11:00:00

Date modified: 2016-10-30 06:22:55

GFP/RFP reporter (Reverse/Forward) with random 500bp spacer

Details

• Types: DnaRegion
• Roles: Reporter; engineered_region
• Sequences: BBa_K2145107_sequence (Version 1)

Description

This plasmid contains a GFP and an RFP reporter separated by a 532-bp spacer containing three gRNA binding sites for dCas. The reporter is designed to demonstrate the effect, if any, of binding a dCas "clamp" (or a random sequence in) between two genes (the idea being that such a clamp can limit propagation of supercoiling between the genes, functionally isolating them).

Notes

Constructed with Golden Gate Assembly.

Source

Constructed from BBa_J04450, BBa_I13522, and a spacer derived from a concatenation of several non-functional plasmid backbone sequences.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I13522	1622,2558	engineered_region, feature/BioBrick
500 bp spacer	1118,1617	sequence_feature, feature/misc
BBa_J04450	45,1113	feature/BioBrick, engineered_region

Other Properties

• igem#experience: Issues
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2145107/1

Member of these Collections

• iGEM Parts Registry