Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Katie Maruna
Date created: 2016-10-17 11:00:00
Date modified: 2016-10-19 03:12:21
This part contains 2 fluorescent protein coding sites (RFP and GFP) with a spacer
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_K2145123_sequence (Version 1) |
Description
This part contains two fluorescent protein coding sites (RFP and GFP) and was assembled using Golden Gate Assembly. In order to make the plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) along with a 500 base pair "spacer" part derived from different backbones, and a pSB1C3 backbone assembled to make the final part. It can be used to express both GFP and RFP proteins.Notes
The plasmid was assembled using Golden Gate Assembly.Source
The RFP part is from BBa_J04450 and the GFP part is from BBa_I13522, with a pSB1C3 backbone, and the "spacer" from a number of different plasmid backbones.| Sequence Annotation | Location | Component / Role(s) |
| BBa_I13522 500 bp spacer BBa_J04450 | 45,981 986,1485 1490,2558 | engineered_region feature/BioBrick feature/misc sequence_feature feature/BioBrick engineered_region |