GG101

BBa_K2145126 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2145126
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Katie Maruna, Laura Chen, Melody Wu
Date created: 2016-10-18 11:00:00
Date modified: 2016-10-30 06:23:36

GFP/RFP reporter (Forward/Reverse) with random 500bp spacer



Types
DnaRegion

Roles
engineered_region

Reporter

Sequences BBa_K2145126_sequence (Version 1)

Description

This part contains two fluorescent protein coding sites (RFP and GFP) and was assembled using Golden Gate Assembly. In order to make the plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) along with a 500 base pair "spacer" part derived from different backbones, and a pSB1C3 backbone assembled to make the final part. It can be used to express both GFP and RFP proteins.

Notes

This plasmid was assembled using Golden Gate Assembly

Source

The RFP part is from BBa_J04450 from the iGEM registry and the GFP part is from BBa_I13522 from the iGEM registry. The backbone is a pSB1C3 backbone and there is a spacer between the RFP part and GFP part derived from different plasmid backbones.

Sequence Annotation Location Component / Role(s)
BBa_J04450
500 bp spacer
BBa_I13522
45,1113
1118,1617
1622,2558
feature/BioBrick engineered_region
sequence_feature feature/misc
feature/BioBrick engineered_region
igem#experience
Issues
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K2145126/1