Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Katie Maruna, Laura Chen, Melody Wu
Date created: 2016-10-18 11:00:00
Date modified: 2016-10-30 06:24:46
GFP/RFP reporter (Reverse/Reverse) with random 500bp spacer
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K2145127_sequence (Version 1) |
Description
This part contains two fluorescent protein coding sites (RFP and GFP) and was assembled using Golden Gate Assembly. In order to make the plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) along with a 500 base pair "spacer" part derived from different backbones, and a pSB1C3 backbone assembled to make the final part. It can be used to express both GFP and RFP proteins.Notes
This plasmid was assembled using Golden Gate Assembly.Source
The RFP part is from BBa_J04450 from the iGEM registry and the GFP part is from BBa_I13522 from the iGEM registry. The backbone is a pSB1C3 backbone and there is a spacer between the RFP part and GFP part derived from different plasmid backbones.| Sequence Annotation | Location | Component / Role(s) |
| BBa_J04450 500 bp spacer BBa_I13522 | 45,1113 1118,1617 1622,2558 | engineered_region feature/BioBrick sequence_feature feature/misc engineered_region feature/BioBrick |