BBa_K2148013

BBa_K2148013 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2148013
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chern Juin Ong, Alex Mayorov, Mie Monti
Date created: 2016-10-10 11:00:00
Date modified: 2016-10-13 02:18:24

Cas9 gene



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K2148013_sequence (Version 1)

Description

This is the coding DNA sequence (CDS) for the Streptococcus pyogenes Cas9 protein. There is a Gly-Ser fusion linker at the end of the sequence to allow for the possibility of forming fusion proteins for verification purposes.

Notes

The Gly-Ser linker is advised by Dr N. Patron (Earlham Institute)

Source

Streptococcus pyogenes

Sequence Annotation Location Component / Role(s)
Phytobrick fusion site
start
cds
GG as linker and to avoid framshift
Phytobrick fusion site
1,4
2,4
5,4105
4106,4107
4108,4111
feature/BioBrick engineered_region
feature/start start_codon
CDS feature/cds
feature/misc sequence_feature
feature/BioBrick engineered_region
igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K2148013/1