BBa_K2149002

BBa_K2149002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2149002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Aline Larissa Gon??alves

Date created: 2016-10-12 11:00:00

Date modified: 2016-10-13 07:54:41

HPT (Homogentisate phytyltransferase)

Details

• Types: DnaRegion
• Roles: Translational_Unit; engineered_region
• Sequences: BBa_K2149002_sequence (Version 1)

Description

The gene homogentisate phytyl transferase (HPT) taken from Synechocystis sp. is involved in the synthesis of tocotrienol and tocopherol. This enzyme has the ability to catalyze the reaction between the recently formed phytylpyrophosphate (PPP) and homogentisic acid(HGA).

Notes

The gene was synthesized with thirty nucleotides in its ends to do a Gibson Assembly overlap. Its design takes into consideration the RBS and the coding sequence.

Source

HPT was taken from Synechocystis sp. (accession number K09833). This gene is the unique transferase
of these organisms able to combine phytylpyrophosphate (PPP) or geranylgeranylpyrophosphate (GGPP) with homogentisate (HGA).

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_B0034	30,42	ribosome_entry_site, feature/rbs
K2149002	43,969	CDS, feature/cds

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2149002/1

Member of these Collections

• iGEM Parts Registry