| Types | DnaRegion
|
| Roles | engineered_region
Reporter
|
| Sequences | BBa_K2150024_sequence (Version 1)
|
Description
The first promoter is constitutively on and can express tetR, a protein that can repress the downstream promoter pTet in the absence of tetracycline(Tc). As a result, no GFP is expressed and no fluorescence can be detected. However, in the presence of Tc, tetR is repressed and repression over pTet is removed. This leads to the expression of GFP and emission of green fluorescence. This part can be used to verify the tet-on/tet-off system.
Notes
This part is used for logic verification. Whether green fluorescence is observed serves as an indicator whether the system works. In real use, substitute GFP for target gene.
Source
pCon from BBa_J23119, RBS from BBa_B0034, tetR from BBa_C0040, DT from BBa_B0015,PTet from BBa_R0040, GFP from BBa_E0040.
