BBa_K215251

BBa_K215251 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K215251
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alex Leone
Date created: 2009-09-29 11:00:00
Date modified: 2015-05-08 01:11:30

Lpp + 5tmr OmpA + GS Linker + TEV site + NheI Site



    • Details

    Types
    DnaRegion

    Roles
    Coding

    CDS

    Sequences BBa_K215251_sequence (Version 1)

    Description

    This is a fusion protein that displays your favorite gene inserted into the NheI site. See BBa_K215250 for 1tmr OmpA.

    Notes

    Anything inserted into the NheI shouldn't have a start codon and gene should still be in frame.

    Source

    Harvard iGEM 2006: BBa_J36848. We added a GS Linker and TEV site.

    Sequence Annotation Location Component / Role(s)
    BBa_J36835 - Lpp Signaling Peptide
    BBa_J36837 - 5tmr OmpA
    GS Linker - GGGSGGGSGGG
    TEV Site - ENLYFQG
    NheI
    		1,87
    94,435
    436,468
    469,489
    490,495
    		feature/BioBrick engineered_region
    feature/BioBrick engineered_region
    sequence_feature feature/misc
    sequence_feature feature/misc
    sequence_feature feature/misc

    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K215251/1
     

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