BBa_K215260

BBa_K215260 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K215260

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Alex Leone

Date created: 2009-09-30 11:00:00

Date modified: 2015-05-08 01:11:30

Lpp + 1tmr OmpA + GS Linker + TEV site + WT Streptavidin + 6His

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K215260_sequence (Version 1)

Description

This is a fusion protein that combines Lpp, OmpA, and WT streptavidin.

See BBa_K215210 for the full induceable WT streptavidin dispaly system.

Notes

See BBa_K215200 for the method used to put WT streptavidin in the OmpA surface display construct.

Source

BBa_K215200 + BBa_J36841

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J36835 - Lpp Signaling Peptide	1,87	feature/BioBrick, engineered_region
BBa_J36836 - 1tmr OmpA	94,156	feature/BioBrick, engineered_region
GS Linker - GGGSGGGSGGG	157,189	sequence_feature, feature/misc
TEV Site - ENLYFQG	190,210	sequence_feature, feature/misc
BBa_J36841 - WT Streptavidin w/o start codon	217,621	feature/BioBrick, engineered_region

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K215260/1

Member of these Collections

• iGEM Parts Registry