BBa_K2160000

BBa_K2160000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2160000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Emma Finlayson-Trick

Date created: 2016-10-13 11:00:00

Date modified: 2016-10-28 10:11:34

Beta-1,4-D-glycosidic bonds in cellulose -> cellulobiose

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K2160000_sequence (Version 1)

Description

The endoglucanase enzyme was taken from the thermophillic bacteria Ruminiclostridium thermocellum. The endoglucanase enzyme cleaves internal Beta-1,4-D-glycosidic bonds in the cellulose crystal to release the disaccharide cellulobiose.

Notes

Carefully designed primers containing restriction enzyme cut sites so that none of the sites were found in the endoglucanase nucleotide sequence or the biobrick vector.

Source

Ruminiclostridium thermocellum nucleotide sequence

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
ATG	17,19	feature/start, start_codon
Shine-delgarno sequence	1,8	feature/rbs, ribosome_entry_site
ORF	17,1048	CDS, feature/cds

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2160000/1

Member of these Collections

• iGEM Parts Registry