Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Edinburgh iGEM 2009
Date created: 2009-08-03 11:00:00
Date modified: 2015-05-08 01:11:30
TNT Receptor TNT-R1
| Types | DnaRegion |
| Roles | engineered_region Translational_Unit |
| Sequences | BBa_K216002_sequence (Version 1) |
Description
TNT.R1 has fair affinity (340nM) for trinitrotoluene (TNT)that is not a naturally occurring compound. TNT.R1 is less thermostable than the wildtype receptor. As such, it is suggested that the receptor be used shortly after expression.TNT receptor 1 (TNT.R1) is a computationally-derived ligand receptor specific for TNT. The ribose-binding pocket of ribose-binding protein, a member of the E. coli periplasmic binding protein (PBP) family, was reconfigured so that the receptor recognizes TNT instead of the wild-type ligand.
Notes
This part is cloned into BioBrick vector pSB1A2.Source
Computationally-derived ligand receptor.Looger, L. L., Dwyer, M. A., Smith, J. J. and Hellinga, H. W. (2003). Computational design of receptor and sensor proteins with novel functions. Nature 423: 185 - 190.