BBa_K216005

BBa_K216005 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K216005
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Edinburgh iGEM 2009
Date created: 2009-09-24 11:00:00
Date modified: 2015-05-08 01:11:30

PyeaR promoter, responsive to nitrate, nitrite and nitric oxide



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_K216005_sequence (Version 1)

Description

PyeaR promoter. This is the promoter of the Escherichia coli yeaR/yoaG operon (see Lin, H.-Y., Bledsoe, P.J., and Stewart, V. 2007. Activation of yeaR-yoaG operon transcription by the nitrate-responsive regulator NarL is independent of oxygen-responsive regulator Fnr in Escherichia coli K-12. J. Bacteriol. 189, 7539-7548). Unlike other E. coli promoters responding to nitrate and nitrite, this promoter is not repressed under aerobic conditions.

Notes

No special considerations.

Source

BioBricked from E. coli K-12 genomic DNA by the Edinburgh 2009 iGEM team.

Sequence Annotation Location Component / Role(s)
NarL-binding region
NsrR-binding region
promoter -35 region
promoter -10 region
50,65
58,80
66,71
89,94
feature/binding non_covalent_binding_site
non_covalent_binding_site feature/binding
non_covalent_binding_site feature/binding
non_covalent_binding_site feature/binding
igem#experience
Works
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K216005/1