Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Edinburgh iGEM 2009
Date created: 2009-10-15 11:00:00
Date modified: 2015-05-08 01:11:30
bacterial luciferase LuxAB of Xenorhabdus luminescens
| Types | DnaRegion |
| Roles | Translational_Unit engineered_region |
| Sequences | BBa_K216008_sequence (Version 1) |
Description
This part includes the coding sequences of luxA and luxB of Xenorhabdus luminescens, with their native ribosome binding sites. The dimeric bacterial luciferase LuxAB produces light in the presence of oxygen, FMNH2, and a long chain aldehyde. The aldehyde can be provided by the luxCDE gene products, but if these are not present, n-decanal can be added to cultures.Notes
No special considerations. Please note that preliminary sequencing suggests that there may be a problem with the SpeI site of the construct submitted. If this is confirmed, we will submit a revised version and remove this note.Source
Amplified from genomic DNA of Xenorhabdus luminescens.| Sequence Annotation | Location | Component / Role(s) |
| rbs start luxA end luxA luxA coding sequence rbs start luxB end luxB luxB coding sequence | 15,20 27,29 1107,1109 27,1109 1112,1117 1124,1126 2105,2107 1124,2107 | ribosome_entry_site feature/rbs feature/start start_codon feature/stop stop_codon CDS feature/cds ribosome_entry_site feature/rbs feature/start start_codon feature/stop stop_codon feature/cds CDS |