BBa_K216017

BBa_K216017 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K216017

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Edinburgh iGEM 2009

Date created: 2009-10-20 11:00:00

Date modified: 2015-05-08 01:11:30

luxCDE genes of Xenorhabdus luminescens (aldehyde biosynthesis)

Details

• Types: DnaRegion
• Roles: Translational_Unit; engineered_region
• Sequences: BBa_K216017_sequence (Version 1)

Description

This part is an artificial operon consisting of the luxCDE genes of the bioluminescent bacterium Xenorhabdus luminescens Hb. The luxCDE gene products synthesise the aldehdye substrate for the luciferase LuxAB.

Notes

The normal operon structure is luxCDABE. This part is derived from an earlier (non-BioBrick) project, in which the luxCD genes were fused to luxE so that the aldehyde biosynthesis machinery could be induced separately from the luciferase. An XbaI site must be removed from within luxD.

Source

Amplified from genomic DNA of Xenorhabdus luminescens Hb (NCIMB 12670).

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
luxC	22,1464	CDS, feature/cds
luxD	1476,2399	CDS, feature/cds
luxE	2455,3560	feature/cds, CDS
rbs	2440,2444	ribosome_entry_site, feature/rbs
rbs	1466,1468	feature/rbs, ribosome_entry_site
rbs	11,16	ribosome_entry_site, feature/rbs

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K216017/1

Member of these Collections

• iGEM Parts Registry