Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Arunavo Chakraborty
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-13 02:44:12
pBAD/araC - N(6xHis) Ag43
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K2168000_sequence (Version 1) |
Description
This part encodes E coli protein Ag43, with an additional 6xHis tag for easy purification and quantification. Ag43 expression is known to cause E coli cells to adhere together, and the 6xHis tag was added to allow for convenient study of variation of E coli aggregation with respect to Ag43 expression levels.Notes
Ag43 is an outer membrane E coli protein, so it has a N-terminal 52 amino acid long signal peptide sequence, which is cleaved after Ag43 pro-protein is transported into the periplasmic space. Since strong dependence has been demonstrated on the signal peptide cleavage on the signal peptide-protein junction, the 6xHis tag should be added after Ala53, the residue immediately after the signal peptide sequence.Source
This comes from Gibson assembly of fragments from a previous biobrick, BBa_K1352000 by Team:Aberdeen_Scotland, 2014.| Sequence Annotation | Location | Component / Role(s) |
| araC pBAD B0034 Ag43(PstI sites removed) 6xHis tag Double terminator (B0015) | 1,879 906,1190 1219,1230 1237,4374 1396,1413 4383,4511 | CDS feature/cds promoter feature/promoter ribosome_entry_site feature/rbs feature/cds CDS tag feature/tag feature/BioBrick engineered_region |