BBa_K2172002

BBa_K2172002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2172002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Bowen Xiao

Date created: 2016-10-13 11:00:00

Date modified: 2016-10-19 08:15:40

Tac Promoter-RBS-GST-Thrombin Protease-TEV-GFP-

Details

• Types: DnaRegion
• Roles: engineered_region; Intermediate
• Sequences: BBa_K2172002_sequence (Version 1)

Description

This part contains SmCPS1 gene, TEV cleavage site and GFP. It can be used to construct further parts that can synthesize SmCPS1.

Notes

Two extra sticky ends are added to the gene when it is cloned via PCR. They are needed so that the part can be loaded onto other vectors. Site-directed mutagenesis is performed to alter the triplets identical to the sequence of restriction enzymes used to remove the part from the vector.

Source

The source of the part is the vector pGEX-KG and Salvia miltiorrhiza

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2172002/1

Member of these Collections

• iGEM Parts Registry