| Types | DnaRegion
|
| Roles | CDS
Coding
|
| Sequences | BBa_K2172005_sequence (Version 1)
|
Description
This part is a gene circuit that can be used to test whether it is being expressed or not. It comprises of a ribosome binding site (RBS) for ribosomes to bind, a GST label for purification, a thrombin restriction enzyme cleavage site plus a TEV restriction enzyme cleavage site (for adding other segments) and a GFP detection unit. When transformed into bacteria, e.g. E. coli, it can be used to test whether the bacteria are expressing the part not. Other parts, such as the gene SmCPS1, can be inserted into this part via digestion on the thrombin restriction enzyme cleavage site and the TEV site.
Notes
Two extra sticky ends are added to the gene when it is cloned via PCR. They are needed so that the part can be loaded onto other vectors. Site-directed mutagenesis is performed to alter the triplets identical to the sequence of restriction enzymes used to remove the part from the vector.
Source
The source of the part is the vector pGEX-KG.
