Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Miranda Halle
Date created: 2016-07-24 11:00:00
Date modified: 2016-07-25 03:22:32
yeGFP Reporter for LexA
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_K2176000_sequence (Version 1) |
Description
This part contains a yeast-optimized GFP (yeGFP) coding sequence, flanked by an ADH1 terminator and a Cyc1 promoter. This promoter is under the control of a LexA operator, which only activates the promoter in the presence of the LexA protein. Thus, cells containing this construct will express GFP only in the presence of LexA.Notes
The GFP used in this construct was optimized for yeast before synthesis. Additionally, a tag was added at the end to make degradation faster.The number of repeats of the LexA operator system was decided based on a previous iGEM part.
Source
The entire sequence for this part was synthesized as G-blocks and assembled via Gibson Assembly.| Sequence Annotation | Location | Component / Role(s) |
| yeGFP CLNpest Degradation Tag | 431,1137 1145,1681 | CDS feature/protein feature/tag tag |