BBa_K2184001

BBa_K2184001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2184001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Margalit Mualem

Date created: 2016-09-18 11:00:00

Date modified: 2016-09-27 05:58:52

Guid RNA for grapefroit flavor

Details

• Types: DnaRegion
• Roles: Protein_Domain; polypeptide_domain
• Sequences: BBa_K2184001_sequence (Version 1)

Description

1. The basic assumption of the project is based on the fact that there is a direct link between particular taste affection and the number of receptors for that taste. For this reason, the ability to control the polymorphism of taste receptors may be useful in many ways to human society
2. Guide RNA is a hundred base-long molecule with a unique two dimensional structure which binds Cas9 and guides it to a dsDNA sequence complementary to 21-22 base pairs on the 5' end of the molecule.
3. This molecule was specially designed to be able to identify SNPs in grapefroit flavor and mediate CRISPR editing of the non-taste allele only.

Notes

This molecule was specially designed to be able to identify SNPs in grapefroit flavor and mediate CRISPR editing of the non-taste allele only.
3. TAS2R19 rs10772420-SNPs in grapefroit flavor with guid RNA
TAAAATGTTCCAGACACCATCAGTTTGTTTTCTGCTAGAAGACCCACGATGCTCCCCTTGTGAATCTATGGAGTTGAGGGTTTCTCTTCTTTCACTCAGC[T/C]TGTCATCTGCCACAAAACTGAAAGAAAGGTCTGTTTTAGCTTCCTACTTCCCATAATCAGGATGAATGAGTGGAATGAAGGATACATGATTGCAACAGTT

Source

GTCATCTGCCACAAAACTGA- A syntethic guid RNA

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2184001/1

Member of these Collections

• iGEM Parts Registry