Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Margalit Mualem
Date created: 2016-09-24 11:00:00
Date modified: 2016-09-25 10:02:53
Non taster PTC - TAS2R38 rs713598
| Types | DnaRegion |
| Roles | sequence_feature DNA |
| Sequences | BBa_K2184020_sequence (Version 1) |
Description
Includes a unique characterization of the composition of taste receptor alleles for the five different tastes. This part will be carried out for each subject. Characterization will be carried out using Taqman technology with the FLUIDIGM systemWe selected 15 SNPs (Single Nucleotide Polymorphism) in favor of this reaction. Each of the SNP???s that were selected enables identification of a certain taste out of the five known senses of taste, Using SNP segments ??? the Taqman reaction makes it possible to identify the presence of a specific allele tested for a specific taste. Whenever a sample DNA is tested a presence of a specific allele is checked by one of the 15 SNP's we receive a fluorescence signal due to the release of the PROBE and vice versa. 67 DNA samples were tested using this method (samples were collected from 67 students and teachers from the school). Each sample was tested to check the presence of alleles to identify different tastes in accordance to the sequence of the SNP.