Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Angeles Hueso Gil
Date created: 2009-10-20 11:00:00
Date modified: 2015-05-08 01:11:31
project of a Mid1 gene disrupted from 489 to 2021 nt
| Types | DnaRegion |
| Roles | Project engineered_region |
Description
The gene of that part was one of our negative controls. We have used it to make sure our luminiscent peaks made by our wt strains were not an artifact. We can see the behaviour of that kind of mutant in the grapfic showed below:[[Image:Graf compar.jpg]]
Notes
1 gaattcatat cagtgtccag gacctctacc caaggcctta catcccactg aaaaaccaaa61 tcgttctcag agatactcag tctatattcc caattttcag aggcgtcgga actggacaga
121 ggttgctccg tagtggaatt cactagctca gcgaccacat aaagatttga atacgtggag
181 gtagcgttcg agctagaaga ctgcactgga ctgatggcta atgcttggat gtagccatcc
241 tgaaaaactg cactttgacc tatatttggg ttctctaaaa ccgtttcatt aaaagagtag
301 tatattgtta aatccacatc tgatctattc atgggcattt ggcatatatt gccgctaata
361 aacattaaca cttcatacgt gggagcaaac ccataaccgg aggcttctgc gtcaatggta
421 aatacaaaac tgtctttttt gcccgctgtt atgttagata agatcggggt ccattcataa
481 atgctatcc------------------------------------------
gggcagcgcg gtcattggcg
2041 ccatcaaggc cagcttcgag aacgaaactg ttaaagccaa cgtataatcg acgcaaatat
2101 gtatagatac aatatgtaca gaacaactgc attgtgcaat ataacaacat aacacaacgc
2161 ccagaacgaa ataaaaaaaa taaaagaaat agatgaaagc attttcaatt tgcataccgg
2221 aaaccgtaaa tcaattggcg tctagctaac aactgagaat gcgaatcgcc aaattgttac
2281 agaaagtagc attccgttac gtgatctgta ctttaacctc ttggacgtaa agaatggcag
2341 aactctggct ctagtgttct gcgaatgcca gatcgggaaa tatttcccaa aagggcaaca
2401 gcggcacgaa caagaatttc gtgttaaaaa tcgcgtccgg cgcgaaattt ttcgcggagg
2461 catgcgacgc aaaagcgact cgaaatgtcg ggagccaaat gaggctacaa ggctgtgggc
2521 agattttggc gttattatgg agaataaaag gaatggtagc ttccatatag cgataggaaa
2581 acatatataa ggtcaatagg cctacatggt aatgggattg ataagcttgt ccttaactct
2641 tgtgtcttgg aattactatt gcgtacatca gcaatcatcc attgtcaaga acaaaaacga
2701 taacaaaaac aatcaattat acaaataaca gtaaagtaat aaa